ABSTRACT
Temulawak (Curcuma xanthorrhiza Roxb.) belongs to the family Zingiberaceae, has been empirically used as herbal medicine. The research aimed to evaluate three promising lines of Temulawak based on their high bioactive contents (xanthorrhizol and curcuminoid) and its in vitro bioactivity (antioxidant and toxicity) and obtain information agrobiophysic environmental condition which produced high bioactive compounds. The xanthorrhizol and curcuminoid contents were measured by HPLC. In vitro, antioxidant and toxicity were determined by DPPH (1,1-diphenyl-2-picryl-hydroxyl) method and BSLT (Brine Shrimp Lethality Test). The result showed that good line A produced the highest yield of bioactive and bioactivity, i.e., 0.157 and 0.056 g plant-1 of xanthorrizol and curcuminoid, respectively. The IC of antioxidant activity was 65.09 mg L-1, and the LC of toxicity was 69.05 mg L-1. In this study, Cipenjo had the best temulawak performance than the two other locations. According to the agrobiophysic parameters, Cipenjo environmental condition was suitable for temulawak cultivation with temperature 28-34 ºC, rainfall ± 223.97 mm year-1, and sandy clay soil.
Keywords: antioxidant, curcuminoid, promising lines, temulawak, xanthorrhizol
source: http://journal.ipb.ac.id/
